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Ecosystem

  • Installation
  • Workflow
    • Package Design
    • scPortrait Projects
    • Config Files
    • SpatialData
    • Segmentation
    • Single-cell image datasets
    • Featurization Methods
  • Tutorials
    • A Walk Through The scPortrait Ecosystem
    • Different segmentation workflows
    • Fine Tuning single-cell image extraction
    • Deep learning with scPortrait
    • Automatically parse image files from Opera Phenix imaging experiments
    • Stitch individual tif files into whole-slide images

Code Examples

  • Code Snippets
  • Example Projects
    • Sharded Cellpose Segmentation
    • Sharded Cellpose Segmentation on human protein atlas images

Module API

  • pipeline
    • project
    • segmentation
    • segmentation workflows
    • extraction
    • Featurization
    • selection
  • processing
  • plotting
    • scportrait.plotting.add_scalebar
    • scportrait.plotting.colorize
    • scportrait.plotting.generate_composite
  • io
  • tools
    • parse
    • stitch
    • ml
    • h5sc
    • sdata
  • .rst

pipeline

pipeline#

  • project
  • Project
    • Project.config
    • Project.nuc_seg_name
    • Project.cyto_seg_name
    • Project.sdata_path
    • Project.filehander
    • Project.DEFAULT_IMAGE_DTYPE
    • Project.DEFAULT_SEGMENTATION_DTYPE
    • Project.DEFAULT_SINGLE_CELL_IMAGE_DTYPE
    • Project.sdata
    • Project.update_featurization_f()
    • Project.print_project_status()
    • Project.view_sdata()
    • Project.plot_input_image()
    • Project.plot_he_image()
    • Project.plot_segmentation_masks()
    • Project.plot_single_cell_images()
    • Project.load_input_from_array()
    • Project.load_input_from_tif_files()
    • Project.load_input_from_omezarr()
    • Project.load_input_from_dask()
    • Project.load_input_from_sdata()
    • Project.complete_segmentation()
    • Project.extract()
    • Project.select()
  • segmentation
    • Segmentation
    • ShardedSegmentation
  • segmentation workflows
    • WGASegmentation
    • ShardedWGASegmentation
    • DAPISegmentation
    • ShardedDAPISegmentation
    • DAPISegmentationCellpose
    • ShardedDAPISegmentationCellpose
    • CytosolSegmentationCellpose
    • ShardedCytosolSegmentationCellpose
    • CytosolOnlySegmentationCellpose
    • ShardedCytosolOnlySegmentationCellpose
    • CytosolSegmentationDownsamplingCellpose
    • ShardedCytosolSegmentationDownsamplingCellpose
    • CytosolOnlySegmentationDownsamplingCellpose
    • ShardedCytosolOnlySegmentationDownsamplingCellpose
  • extraction
    • HDF5CellExtraction
  • Featurization
    • MLClusterClassifier
    • EnsembleClassifier
    • CellFeaturizer
    • ConvNeXtFeaturizer
  • selection
    • LMDSelection

previous

Cellpose Segmentation on human protein atlas images

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By Sophia Mädler and Niklas Schmacke

© Copyright 2026 Sophia Mädler and Niklas Schmacke.